Cell-Free Protein Synthesis Kit Max (Lyophilized)

• Description:

  The Cell-Free Protein Synthesis Kit is a product for in vitro protein synthesis based on E. coli lysate. Coupled transcription/translation systems contain an RNA polymerase and the necessary cellular machinery needed to direct protein synthesis (e.g., ribosomes, translation factors and tRNAs) . Supplements such as amino acids, an energy source and NTPs complete the system. Proteins are expressed using DNA or RNA as templates. Cell-free protein expression, independent of viable cells, enables rapid, flexible and high-yield protein expression, with numerous advantages over traditional recombinant expression systems: 1. Time saving. Express target proteins in 1~2 hours, with peak yield achieved in 8~24 hours. 2. High protein expression level, up to more than 3 mg/ml. 3. Easy operation, Add DNA or RNA templates to the reaction system, and the reaction can be performed using 96-well plates or centrifuge tubes. Note: The product is suitable for expressing diverse proteins, particularly disulfide-rich proteins.

• Assay Protocol:

  http://en.best-enzymes.com/storage/upload/files/directions/11danbaiyanjiuxilie/EG25304.pdf

• Components:

  • Cell-free system solution A Max (Lyophilized) : 1 vial (300 μl after reconstitution) • Cell-free system solution B (Lyophilized) : 1 vial (600 μl after reconstitution) • CFPS-Control Plasmid: 2 μg

• Shipping Conditions:

  2~8°C

• Storage Conditions:

  4°C transportation. Store at -20°C. Valid for 12 months. After reconstitution, store at -20°C for no more than 1 month, and at -80°C for no more than 12 months.

• Shelf Life:

  1 Year