Cell-Free Protein Synthesis Kit (Kcr)

• Description:

  Cell-Free Protein Synthesis Kit is an extract-based transcription/translation system derived from E. coli cells. It enables the site-specific incorporation of the Nε-crotonyl-L-lysine (Kcr) into the target protein by utilizing the amber stop codon TAG in the DNA sequence. The kit contains three additional components: the noncanonical amino acid Kcr, tRNA, and aminoacyl-tRNA synthetase. Dosage can be optimized according to requirements. Note: This product under this catalog number enables the expression of disulfide bond-rich proteins.

• Assay Protocol:

  http://en.best-enzymes.com/storage/upload/files/directions/11danbaiyanjiuxilie/EG25332.pdf

• Components:

  • Cell-free system solution A: 300 μl • Cell-free system solution B: 400 μl • CFPS-Control Plasmid: 2 μg • Nε-crotonyl-L-lysine Kcr (25x) : 60 μl • tRNA (25x) : 60 μl • Aminoacyl-tRNA synthetase (50x) : 40 μl

• Storage Conditions:

  Store at -80°C. Valid for 12 months. Dry ice transportation. After opening, all components should be stored at -80°C. To avoid multiple freeze-thaw cycles, Solution A and Solution B can be aliquoted according to reaction volume, snap-frozen in liquid nitrogen, and then stored at -80°C.