Bst II DNA Polymerase (Large Fragment, Glycerol Free)

• Description:

  Bst II DNA Polymerase (Large Fragment, Glycerol Free) is a modified version of Bacillus stearothermophilus DNA polymerase. It has been engineered and expressed in Escherichia coli. This product exhibits strong 5'→3' DNA polymerase activity and strand displacement activity but lacks 5'→3' exonuclease activity, and the optimal reaction temperature is 65°C. lt is suitable for various isothermal amplification reactions, such as Loop-Mediated lsothermal Amplification (LAMP), Rolling Circle Amplification (RCA), Helicase-Dependent Amplification (HDA), etc. Compared to the wild-type enzyme, Bst II DNAPolymerase (Large Fragment, Glycerol Free) has a faster amplification rate, stronger dUTP tolerance, salt tolerance, and improved stability. This product is glycerol-free and can be used for the preparation of various lyophilized reagents. Avoid repeated freeze-thaw.

• Assay Protocol:

  http://en.best-enzymes.com/storage/upload/files/directions/01juhemei/EG23102-1.pdf

• Concentration:

  8 U/μL

• Components:

  • Bst II DNA Polymerase (Large Fragment, Glycerol Free) (8 U/μl) : 200 μl • 10x Bst II Reaction Buffer: 1 ml • MgSO4 (100 mM) : 1 ml

• Shipping Conditions:

  2~8°C

• Storage Conditions:

  -20°C

• Shelf Life:

  1 Year