Bst II DNA Polymerase (Large Fragment)

• Description:

  Bst II DNA Polymerase (Large Fragment) is a modified form of Bst DNA polymerase obtained by truncating the 5' → 3' exonuclease domain. Through directed evolution and recombinant expression in Escherichia coli , this enzyme has been engineered. It possesses robust 5' → 3' DNA polymerase activity and strand displacement activity. The optimal reaction temperature for this enzyme is 65°C, and it is suitable for various isothermal amplification reactions.Compared to the wild-type enzyme, Bst II DNA Polymerase (Large Fragment) exhibits faster amplification rates, enhanced tolerance to dUTP and salt, as well as improved stability.

• Assay Protocol:

  http://en.best-enzymes.com/storage/upload/files/directions/01juhemei/EG23101-1.pdf

• Concentration:

  8 U/μL

• Components:

  • Bst II DNA Polymerase (Large Fragment) (8 U/μl) : 1 ml • 10x Bst II Reaction Buffer: 3x1 ml • MgSO4 (100 mM) : 3x1 ml

• Shipping Conditions:

  2~8°C

• Storage Conditions:

  -20°C

• Shelf Life:

  2 Years