Recombinant Myrmecia pilosula M-myrmeciitoxin-Mp2b

• Product Name Alternative:

  DELTA-myrtoxin-Mp1a B chain Short name: Mp1a B chain Pilosin-3 subunit b Short name: Pilosulin-3b

• Abbreviation:

  Recombinant Myrmecia pilosula M-myrmeciitoxin-Mp2b protein

• UniProt:

  P0C023

• Expression Region:

  1-23aa

• Organism:

  Myrmecia pilosula (Jack jumper ant) (Australian jumper ant)

• Target Sequence:

  LIGLVSKGTCVLVKTVCKKVLKQ

• Tag:

  N-terminal GST-tagged and C-terminal 6xHis-tagged

• Type:

  In Stock Protein

• Source:

  Baculovirus

• Field of Research:

  Others

• Relevance:

  Heterodimer protein that may serve both defensive (pain-inducing) and predatory (insecticidal) roles (PubMed:28513074) . Has membrane-disrupting activity and shows induction of non-specific calcium influx into cells, (PubMed:28513074) . Shows broad-spectrum activity against a diverse range of bacteria, and cell lines, as well as hemolytic activity (EC (50) =2.18 µM) (PubMed:28513074) . In vivo, shows moderate insecticidal activity against D.melanogaster and potent anthelmintic activity against the veterinary nematode H.contortus (PubMed:32629771) . In addition, intraplantar injection into mice induces nocifensive behavior and mechanical allodynia (PubMed:28513074) .

• Endotoxin:

  Not test

• Purity:

  Greater than 85% as determined by SDS-PAGE.

• Activity:

  Not Test

• Form:

  Liquid or Lyophilized powder

• Buffer:

  If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Molecular Weight:

  30.8 kDa

• References & Citations:

  "It takes two: dimerization is essential for the broad-spectrum predatory and defensive activities of the venom peptide Mp1a from the jack jumper ant Myrmecia pilosula." Nixon S.A., Dekan Z., Robinson S.D., Guo S., Vetter I., Kotze A.C., Alewood P.F., King G.F., Herzig V. Biomedicines 8:0-0 (2020)

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

• Protein Length:

  Full Length