Recombinant Mouse Beta-defensin 1 (Defb1)

• Product Name Alternative:

  Short name:BD-1 Short name:mBD-1

• Abbreviation:

  Recombinant Mouse Defb1 protein

• Gene Name:

  Defb1

• UniProt:

  P56386

• Expression Region:

  33-69aa

• Organism:

  Mus musculus (Mouse)

• Target Sequence:

  DQYKCLQHGGFCLRSSCPSNTKLQGTCKPDKPNCCKS

• Tag:

  N-terminal 6xHis-SUMO-tagged

• Type:

  In Stock Protein

• Source:

  E.coli

• Field of Research:

  Others

• Relevance:

  Has bactericidal activity.

• Endotoxin:

  Not test

• Purity:

  Greater than 90% as determined by SDS-PAGE.

• Activity:

  Not Test

• Form:

  Liquid or Lyophilized powder

• Buffer:

  If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Function:

  Has bactericidal activity. May act as a ligand for C-C chemokine receptor CCR6. Positively regulates the sperm motility and bactericidal activity in a CCR6-dependent manner. Binds to CCR6 and triggers Ca2+ mobilization in the sperm which is important for its motility.

• Molecular Weight:

  20.1 kDa

• References & Citations:

  "The mouse genome encodes a single homolog of the antimicrobial peptide human beta-defensin 1."Huttner K.M., Kozak C.A., Bevins C.L.FEBS Lett. 413:45-49 (1997)

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

• Protein Length:

  Full Length of Mature Protein