Recombinant Mycobacterium tuberculosis Transporter PPE51 (PPE51)

• Product Name Alternative:

  Proline-proline-glutamate family protein 51; Protein PPE51

• Abbreviation:

  Recombinant Mycobacterium tuberculosis PPE51 protein

• Gene Name:

  PPE51

• UniProt:

  P9WHY2

• Expression Region:

  1-380aa

• Organism:

  Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)

• Target Sequence:

  MDFALLPPEVNSARMYTGPGAGSLLAAAGGWDSLAAELATTAEAYGSVLSGLAALHWRGPAAESMAVTAAPYIGWLYTTAEKTQQTAIQARAAALAFEQAYAMTLPPPVVAANRIQLLALIATNFFGQNTAAIAATEAQYAEMWAQDAAAMYGYATASAAAALLTPFSPPRQTTNPAGLTAQAAAVSQATDPLSLLIETVTQALQALTIPSFIPEDFTFLDAIFAGYATVGVTQDVESFVAGTIGAESNLGLLNVGDENPAEVTPGDFGIGELVSATSPGGGVSASGAGGAASVGNTVLASVGRANSIGQLSVPPSWAAPSTRPVSALSPAGLTTLPGTDVAEHGMPGVPGVPVAAGRASGVLPRYGVRLTVMAHPPAAG

• Tag:

  N-terminal 6xHis-tagged

• Type:

  Developed Protein

• Source:

  E.coli

• Field of Research:

  Others

• Relevance:

  Small molecule-selective channel required for the uptake of nutrients across the outer mycomembrane. Transports glycerol and glucose. Involved in sensitivity to M.tuberculosis growth inhibitory agrichemical 3,3-bis-di (methylsulfonyl) propionamide (3bMP1) . Transports maltose and lactose disaccharides. Involved in sensitivity to bactericidal thio-disaccharide T-6 compound (1,6-anhydro-3-deoxy-4-S- (2,3,4,6-tetra-O-acetyl-beta-D-glucopyranosyl) -D-glycero-hexopyranos-2-ulose) . Transports extracellular trehalose, a component of the cell envelope, and trehalose analog, 6-azido trehalose (6-TreAz), which has antimycobacterial activity.

• Endotoxin:

  Not test

• Purity:

  Greater than 85% as determined by SDS-PAGE.

• Activity:

  Not Test

• Bioactivity:

  Not Test

• Form:

  Liquid or Lyophilized powder

• Buffer:

  If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Molecular Weight:

  44.0 kDa

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

• Protein Length:

  Full Length