Recombinant Canine distemper virus Nucleoprotein (N), partial

• Product Name Alternative:

  Nucleocapsid protein

• Abbreviation:

  Recombinant Canine distemper virus N protein, partial

• Gene Name:

  N

• UniProt:

  P04865

• Expression Region:

  399-523aa

• Organism:

  Canine distemper virus (strain Onderstepoort) (CDV)

• Target Sequence:

  SKTTEDRTIRATGPKQSQITFLHSERSEVANQQPPTINKRSENQGGDKYPIHFSDERLPGYTPDVNSSEWSESRYDTQIIQDDGNDDDRKSMEAIAKMRMLTKMLSQPGTSEDNSPVYSDKELLN

• Tag:

  C-terminal 6xHis-Myc-tagged

• Type:

  Developed Protein

• Source:

  Yeast

• Field of Research:

  Microbiology

• Relevance:

  Encapsidates the genome in a ratio of 1 N per 6 ribonucleotides, protecting it from nucleases. The nucleocapsid (NC) has a helical structure. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. During replication, encapsidation by N is coupled to RNA synthesis and all replicative products are resistant to nucleases (By similarity) .

• Endotoxin:

  Not test

• Purity:

  Greater than 85% as determined by SDS-PAGE.

• Activity:

  Not Test

• Form:

  Liquid or Lyophilized powder

• Buffer:

  If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Molecular Weight:

  17.9 kDa

• References & Citations:

  "Canine distemper terminal and intergenic non-protein coding nucleotide sequences: completion of the entire CDV genome sequence." Sidhu M.S., Husar W., Cook S.D., Dowling P.C., Udem S.A. Virology 193:66-72 (1993)

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

• Protein Length:

  Partial