Recombinant Human Laminin subunit beta-2 (LAMB2), partial

• Product Name Alternative:

  Laminin B1s chain; Laminin-11 subunit beta; Laminin-14 subunit beta; Laminin-15 subunit beta; Laminin-3 subunit beta; Laminin-4 subunit beta; Laminin-7 subunit beta; Laminin-9 subunit beta; S-laminin subunit beta ; S-LAM beta

• Abbreviation:

  Recombinant Human LAMB2 protein, partial

• Gene Name:

  LAMB2

• UniProt:

  P55268

• Expression Region:

  1530-1798aa

• Organism:

  Homo sapiens (Human)

• Target Sequence:

  PDSIEMVATRVLELSIPASAEQIQHLAGAIAERVRSLADVDAILARTVGDVRRAEQLLQDARRARSWAEDEKQKAETVQAALEEAQRAQGIAQGAIRGAVADTRDTEQTLYQVQERMAGAERALSSAGERARQLDALLEALKLKRAGNSLAASTAEETAGSAQGRAQEAEQLLRGPLGDQYQTVKALAERKAQGVLAAQARAEQLRDEARDLLQAAQDKLQRLQELEGTYEENERALESKAAQLDGLEARMRSVLQAINLQVQIYNTCQ

• Tag:

  N-terminal 6xHis-tagged

• Type:

  Developed Protein

• Source:

  E.coli

• Field of Research:

  Cell Adhesion

• Relevance:

  Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during bryonic development by interacting with other Extracellular domain matrix components.

• Endotoxin:

  Not test

• Purity:

  Greater than 90% as determined by SDS-PAGE.

• Activity:

  Not Test

• Form:

  Liquid or Lyophilized powder

• Buffer:

  If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Function:

  Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.

• Molecular Weight:

  33.3 kDa

• References & Citations:

  Human laminin beta2 deficiency causes congenital nephrosis with mesangial sclerosis and distinct eye abnormalities.Zenker M., Aigner T., Wendler O., Tralau T., Muentefering H., Fenski R., Pitz S., Schumacher V., Royer-Pokora B., Wuehl E., Cochat P., Bouvier R., Kraus C., Mark K., Madlon H., Doetsch J., Rascher W., Maruniak-Chudek I. , Lennert T., Neumann L.M., Reis A.Hum. Mol. Genet. 13:2625-2632 (2004)

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

• Protein Length:

  Partial