Recombinant Mouse Protein Wnt-4 (Wnt4)

• Abbreviation:

  Recombinant Mouse Wnt4 protein

• Gene Name:

  Wnt4

• UniProt:

  P22724

• Expression Region:

  23-351aa

• Organism:

  Mus musculus (Mouse)

• Target Sequence:

  SNWLYLAKLSSVGSISEEETCEKLKGLIQRQVQMCKRNLEVMDSVRRGAQLAIEECQYQFRNRRWNCSTLDSLPVFGKVVTQGTREAAFVYAISSAGVAFAVTRACSSGELEKCGCDRTVHGVSPQGFQWSGCSDNIAYGVAFSQSFVDVRERSKGASSSRALMNLHNNEAGRKAILTHMRVECKCHGVSGSCEVKTCWRAVPPFRQVGHALKEKFDGATEVEPRRVGSSRALVPRNAQFKPHTDEDLVYLEPSPDFCEQDIRSGVLGTRGRTCNKTSKAIDGCELLCCGRGFHTAQVELAERCGCRFHWCCFVKCRQCQRLVEMHTCR

• Tag:

  C-terminal 6xHis-tagged

• Type:

  Developed Protein

• Source:

  Baculovirus

• Field of Research:

  Cancer

• Relevance:

  Ligand for members of the frizzled family of seven transmembrane receptors (Probable) . Plays an important role in the embryonic development of the urogenital tract and the lung . Required for normal mesenchyme to epithelium transition during embryonic kidney development . Required for the formation of early epithelial renal vesicles during kidney development . Required for normal formation of the Mullerian duct in females, and normal levels of oocytes in the ovaries . Required for normal down-regulation of 3 beta-hydroxysteroid dehydrogenase in the ovary . Required for normal lung development and for normal patterning of trachael cartilage rings .

• Endotoxin:

  Not test

• Purity:

  Greater than 85% as determined by SDS-PAGE.

• Activity:

  Not Test

• Form:

  Liquid or Lyophilized powder

• Buffer:

  If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Molecular Weight:

  42.3 kDa

• References & Citations:

  "Generation of an allele to inactivate Wnt4 gene function conditionally in the mouse." Shan J., Jokela T., Peltoketo H., Vainio S. Genesis 47:782-788 (2009)

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

• Protein Length:

  Full Length of Mature Protein