Recombinant Human Somatotropin (GH1) (Active)

• Product Name Alternative:

  Growth hormone (GH) (GH-N) (Growth hormone 1) (Pituitary growth hormone)

• Abbreviation:

  Recombinant Human GH1 protein (Active)

• Gene Name:

  GH1

• UniProt:

  P01241

• Expression Region:

  27-217aa

• Organism:

  Homo sapiens (Human)

• Target Sequence:

  FPTIPLSRLFDNAMLRAHRLHQLAFDTYQEFEEAYIPKEQKYSFLQNPQTSLCFSESIPTPSNREETQQKSNLELLRISLLLIQSWLEPVQFLRSVFANSLVYGASDSNVYDLLKDLEEGIQTLMGRLEDGSPRTGQIFKQTYSKFDTNSHNDDALLKNYGLLYCFRKDMDKVETFLRIVQCRSVEGSCGF

• Tag:

  N-terminal 10xHis-tagged and C-terminal Myc-tagged

• Type:

  Active Protein & In Stock Protein

• Source:

  Mammalian cell

• Field of Research:

  Developmental Biology

• Relevance:

  Growth hormone (GH) (GH-N) (Growth hormone 1) (Pituitary growth hormone)

• Endotoxin:

  Less than 1.0 EU/μg as determined by LAL method.

• Purity:

  Greater than 90% as determined by SDS-PAGE.

• Activity:

  Yes

• Bioactivity:

  ①Measured by its binding ability in a functional ELISA. Immobilized GH1 at 1 μg/ml can bind human PRLR (CSB-MP018727HU1), the EC50 of the protein is 60.71-69.65 ng/ml. ②Measured by its binding ability in a functional ELISA. Immobilized GH1 at 1 μg/ml can bind human GHR (CSB-MP009411HU), the EC50 of the protein is 19.28-25.29 ng/ml.

• Form:

  Lyophilized powder

• Buffer:

  Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Molecular Weight:

  27.2 kDa

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

• Protein Length:

  Full Length of Mature Protein