Recombinant Triticum aestivum Ubiquitin-activating enzyme E1 3 (UBA3), partial

• Abbreviation:

  Recombinant Triticum aestivum UBA3 protein, partial

• Gene Name:

  UBA3

• UniProt:

  P31252

• Expression Region:

  429-639aa

• Organism:

  Triticum aestivum (Wheat)

• Target Sequence:

  ESLPTYPLEPQDLKPSNNRYDAQVSVFGSKLQKKMEEANTFVVGSGALGCEFLKNLALMGVSCSSKGKLTITDDDIIEKSNLSRQFLFRDWNIGQAKSTVAATAASAINPSLHIDALQNRACPDTENVFHDTFWEGLDVVINALDNVNARMYMDMRCLYFQKPLLESGTLGAKCNIQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNID

• Tag:

  N-terminal 6xHis-KSI-tagged

• Type:

  Developed Protein

• Source:

  E.coli

• Field of Research:

  Cell Biology

• Relevance:

  Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP.

• Endotoxin:

  Not test

• Purity:

  Greater than 85% as determined by SDS-PAGE.

• Activity:

  Not Test

• Form:

  Liquid or Lyophilized powder

• Buffer:

  If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Molecular Weight:

  38.7 kDa

• References & Citations:

  "Multiple forms of ubiquitin-activating enzyme E1 from wheat. Identification of an essential cysteine by in vitro mutagenesis." Hatfield P.M., Vierstra R.D. J. Biol. Chem. 267:14799-14803 (1992)

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

• Protein Length:

  Partial