Recombinant Human Interleukin-21 (IL21) (Active)

• Product Name Alternative:

  Interleukin-21; IL-21; Za11

• Abbreviation:

  Recombinant Human IL21 protein (Active)

• Gene Name:

  IL21

• UniProt:

  Q9HBE4

• Expression Region:

  23-155aa

• Organism:

  Homo sapiens (Human)

• Target Sequence:

  QGQDRHMIRMRQLIDIVDQLKNYVNDLVPEFLPAPEDVETNCEWSAFSCFQKAQLKSANTGNNERIINVSIKKLKRKPPSTNAGRRQKHRLTCPSCDSYEKKPPKEFLERFKSLLQKMIHQHLSSRTHGSEDS

• Tag:

  C-terminal hFc1-tagged

• Type:

  Active Protein & In Stock Protein

• Source:

  Mammalian cell

• Field of Research:

  Cancer

• Relevance:

  Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG (1) and IgG (3) in B-cells (By similarity) . Implicated in the generation and maintenance of T follicular helper (Tfh) cells and the formation of germinal-centers. Together with IL6, control the early generation of Tfh cells and are critical for an effective antibody response to acute viral infection (By similarity) . May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells (PubMed:11081504, PubMed:15178704) . During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation (By similarity) . {ECO:0000250|UniProtKB:Q9ES17, ECO:0000269|PubMed:11081504, ECO:0000269|PubMed:15178704}.

• Endotoxin:

  Less than 1.0 EU/μg as determined by LAL method.

• Purity:

  Greater than 90% as determined by SDS-PAGE.

• Activity:

  Yes

• Bioactivity:

  1Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis IL21R (CSB-MP7140MOV) at 2 μg/mL can bind Human IL21. The EC50 is 15.01-19.43 ng/mL. 2Measured by its binding ability in a functional ELISA. Immobilized Human IL21R (CSB-MP862061HU) at 2 μg/mL can bind Human IL21. The EC50 is 16.65-19.49 ng/mL. 3Human IL21 captured on Protein A Chip can bind Recombinant Macaca fascicularis IL21R (CSB-MP7140MOV) with an affinity constant of 1.31 nM as detected by MetaSPR Assay (WeSPRTM 200) . 4Human IL21 captured on Protein A Chip can bind Recombinant Human IL21R (CSB-MP862061HU) with an affinity constant of 1.87 nM as detected by MetaSPR Assay (WeSPRTM 200) .

• Form:

  Lyophilized powder

• Buffer:

  Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Molecular Weight:

  44.4 kDa

• References & Citations:

  Cloning and characterization of an isoform of interleukin-21. Rahman M., Nara H., Onoda T., Araki A., Li J., Hoshino T., Asao H. FEBS Lett. 581:4001-4009 (2007)

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

• Protein Length:

  Full Length of Mature Protein