Recombinant Human Asialoglycoprotein receptor 1 (ASGR1) -Detergent (Active)

• Product Name Alternative:

  Asialoglycoprotein receptor 1; ASGP-R 1; ASGPR 1; C-type lectin domain family 4 member H1; Hepatic lectin H1; HL-1

• Abbreviation:

  Recombinant Human ASGR1 protein-Detergent (Active)

• Gene Name:

  ASGR1

• UniProt:

  P07306

• Expression Region:

  1-291aa

• Organism:

  Homo sapiens (Human)

• Target Sequence:

  MTKEYQDLQHLDNEESDHHQLRKGPPPPQPLLQRLCSGPRLLLLSLGLSLLLLVVVCVIGSQNSQLQEELRGLRETFSNFTASTEAQVKGLSTQGGNVGRKMKSLESQLEKQQKDLSEDHSSLLLHVKQFVSDLRSLSCQMAALQGNGSERTCCPVNWVEHERSCYWFSRSGKAWADADNYCRLEDAHLVVVTSWEEQKFVQHHIGPVNTWMGLHDQNGPWKWVDGTDYETGFKNWRPEQPDDWYGHGLGGGEDCAHFTDDGRWNDDVCQRPYRWVCETELDKASQEPPLL

• Tag:

  C-terminal 10xHis-tagged

• Type:

  MP-Detergent Transmembrane Protein & Active Protein & In Stock Protein

• Source:

  Mammalian cell

• Field of Research:

  Cancer

• Relevance:

  Mediates the endocytosis of plasma glycoproteins to which the terminal sialic acid residue on their complex carbohydrate moieties has been removed. The receptor recognizes terminal galactose and N-acetylgalactosamine units. After ligand binding to the receptor, the resulting complex is internalized and transported to a sorting organelle, where receptor and ligand are disassociated. The receptor then returns to the cell membrane surface.

• Endotoxin:

  Less than 1.0 EU/μg as determined by LAL method.

• Purity:

  Greater than 85% as determined by SDS-PAGE.

• Activity:

  Yes

• Bioactivity:

  1Measured by its binding ability in a functional ELISA. Immobilized Human ASGR1 at 2 μg/mL on an Nickel Coated plate can bind Anti-ASGR1 recombinant antibody (CSB-RA002207MA3HU) . The EC50 is 2.715-3.605 ng/mL. 2ASGR1 Recombinant Monoclonal Antibody (CSB-RA002207MA3HU) captured on Protein A Chip can bind Recombinant Human ASGR1 with an affinity constant of 0.716 nM as detected by MetaSPR Assay (WeSPRTM 200) .

• Form:

  Lyophilized powder

• Buffer:

  Lyophilized from a 0.2 μm sterile filtered 50 mM HEPES, 0.15 M NaCl, 0.05% DDM, 0.01% CHS, 6% Trehalose, pH 7.5

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Molecular Weight:

  34.6 kDa

• References & Citations:

  A new splice variant of the major subunit of human asialoglycoprotein receptor encodes a secreted form in hepatocytes. Liu J., Hu B., Yang Y., Ma Z., Yu Y., Liu S., Wang B., Zhao X., Lu M., Yang D. PLoS ONE 5:E12934-E12934 (2010)

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

• Transmembrane Domains:

  1TM

• Protein Length:

  Full Length