Recombinant Bovine Hepatocyte growth factor (HGF) (Active)

• Product Name Alternative:

  Hepatocyte growth factor; Hepatopoietin-A; Scatter factor; SF [Cleaved into: Hepatocyte growth factor alpha chain; Hepatocyte growth factor beta chain]

• Abbreviation:

  Recombinant Bovine HGF protein (Active)

• Gene Name:

  HGF

• UniProt:

  Q76BS1

• Expression Region:

  32-730aa

• Organism:

  Bos taurus (Bovine)

• Target Sequence:

  QKKRRNTLHEFKRSAKTTLIKEDPLLKIKTKKMNTADQCANRCIRNKGLPFTCKAFVFDKARKRCLWFPFNSMSSGVKKEFGHEFDLYENKDYIRNCIIGKGGSYKGTVSITKSGIKCQPWNSMIPHEHSFLPSSYRGKDLQENYCRNPRGEEGGPWCFTSNPEVRYEVCDIPQCSEVECMTCNGESYRGPMDHTETGKICQRWDHQTPHRHKFLPERYPDKGFDDNYCRNPDGKPRPWCYTLDPDTPWEYCAIKMCAHSTMNDTDLPMQTTECIQGQGEGYRGTINTIWNGIPCQRWDSQYPHQHDITPENFKCKDLRENYCRNPDGAESPWCFTTDPNIRVGYCSQIPKCDVSSGQDCYRGNGKNYMGSLSKTRSGLTCSMWDKNMEDLHRHIFWEPDATKLNKNYCRNPDDDAHGPWCYTGNPLIPWDYCPISRCEGDTTPTIVNLDHPVISCAKTKQLRVVNGIPTRTNVGWMVSLKYRNKHICGGSLIKESWILTARQCFPSRNKDLKDYEAWLGIHDVHGRGDEKRKQVLNVTQLVYGPEGSDLVLLKLARPAILDDFVSTIDLPNYGCTIPEKTTCSVYGWGYTGLINSDGLLRVAHLYIMGNEKCSQYHQGKVTLNESEICAGAENIVSGPCEGDYGGPLVCEQHKMRMVLGVIVPGRGCAIPNRPGIFVRVAYYAKWIHKIILTYKAPQL

• Tag:

  C-terminal 10xHis-tagged

• Type:

  Active Protein & In Stock Protein

• Source:

  Mammalian cell

• Field of Research:

  Others

• Relevance:

  Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types (By similarity) . Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization (By similarity) . Activates MAPK signaling following TMPRSS13 cleavage and activation (By similarity) .

• Endotoxin:

  Less than 1.0 EU/μg as determined by LAL method.

• Purity:

  Greater than 95% as determined by SDS-PAGE.

• Activity:

  Yes

• Bioactivity:

  1Measured by its binding ability in a functional ELISA. Immobilized Bovine HGF at 2 μg/mL can bind Anti-HGF recombinant antibody (CSB-RA010327MA1HU) . The EC50 is 38.88-61.07 ng/mL. 2Measured by its binding ability in a functional ELISA.Immobilized Bovine HGF at 2 μg/mL can bind Human MET (CSB-MP012376MO1d9) . The EC50 is 2.471-2.843 ng/mL. 3HGF Recombinant Monoclonal Antibody (CSB-RA010327MA1HU) captured on Protein A Chip can bind Bovine HGF with an affinity constant of 1.73 nM as detected by MetaSPR Assay (WeSPRTM 200) . 4Recombinant Human MET (CSB-MP012376MO1d9) captured on Protein A Chip can bind Recombinant Bovine HGF with an affinity constant of 0.643 nM as detected by MetaSPR Assay (WeSPRTM 200) .

• Form:

  Lyophilized powder

• Buffer:

  Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Molecular Weight:

  81.2 kDa

• References & Citations:

  Bovine hepatocyte growth factor and its receptor c-Met: cDNA cloning and expression analysis in the mammary gland. Yamaji D., Kimura K., Watanabe A., Kon Y., Iwanaga T., Soliman M.M., Ahmed M.M., Saito M. Domest. Anim. Endocrinol. 30:239-246 (2006)

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

• Protein Length:

  Full Length of Mature Protein