Recombinant Human Syndecan-1 (SDC1), partial (Active)

• Product Name Alternative:

  Syndecan-1; SYND1; CD antigen CD138

• Abbreviation:

  Recombinant Human SDC1 protein, partial (Active)

• Gene Name:

  SDC1

• UniProt:

  P18827

• Expression Region:

  23-254aa

• Organism:

  Homo sapiens (Human)

• Target Sequence:

  QIVATNLPPEDQDGSGDDSDNFSGSGAGALQDITLSQQTPSTWKDTQLLTAIPTSPEPTGLEATAASTSTLPAGEGPKEGEAVVLPEVEPGLTAREQEATPRPRETTQLPTTHLASTTTATTAQEPATSHPHRDMQPGHHETSTPAGPSQADLHTPHTEDGGPSATERAAEDGASSQLPAAEGSGEQDFTFETSGENTAVVAVEPDRRNQSPVDQGATGASQGLLDRKEVLG

• Tag:

  C-terminal 10xHis-tagged

• Type:

  Active Protein & In Stock Protein

• Source:

  Mammalian cell

• Field of Research:

  Cancer

• Relevance:

  Cell surface proteoglycan that contains both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix (By similarity) . Regulates exosome biogenesis in concert with SDCBP and PDCD6IP (PubMed:22660413) . Able to induce its own expression in dental mesenchymal cells and also in the neighboring dental epithelial cells via an MSX1-mediated pathway (By similarity) .

• Endotoxin:

  Less than 1.0 EU/μg as determined by LAL method.

• Purity:

  Greater than 95% as determined by SDS-PAGE.

• Activity:

  Yes

• Bioactivity:

  1Measured by its binding ability in a functional ELISA.Immobilized Human SDC1 at 2 μg/mL can bind anti-SDC1 recombinant antibody (CSB-RA020888MA1HU) .The EC50 is 0.7196-0.8309 ng/mL. 2SDC1 Recombinant Monoclonal Antibody (CSB-RA020888MA1HU) captured on Protein A Chip can bind Human SDC1 with an affinity constant of 6.79 nM as detected by MetaSPR Assay (WeSPRTM 200) .

• Form:

  Lyophilized powder

• Buffer:

  Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Molecular Weight:

  25.2 kDa

• References & Citations:

  Shift of syndecan-1 expression from epithelial to stromal cells during progression of solid tumours. Mennerich D., Vogel A., Klaman I., Dahl E., Lichtner R.B., Rosenthal A., Pohlenz H.D., Thierauch K.H., Sommer A. Eur. J. Cancer 40:1373-1382 (2004)

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

• Protein Length:

  Partial