Recombinant Neisseria meningitidis serogroup B Neisseria adhesin A (nadA), partial

• Product Name Alternative:

  NadA variant 1

• Abbreviation:

  Recombinant Neisseria meningitidis serogroup B nadA protein, partial

• Gene Name:

  NadA

• UniProt:

  Q9JXK7

• Expression Region:

  24-306aa

• Organism:

  Neisseria meningitidis serogroup B (strain MC58)

• Target Sequence:

  ATSDDDVKKAATVAIVAAYNNGQEINGFKAGETIYDIGEDGTITQKDATAADVEADDFKGLGLKKVVTNLTKTVNENKQNVDAKVKAAESEIEKLTTKLADTDAALADTDAALDETTNALNKLGENITTFAEETKTNIVKIDEKLEAVADTVDKHAEAFNDIADSLDETNTKADEAVKTANEAKQTAEETKQNVDAKVKAAETAAGKAEAAAGTANTAADKAEAVAAKVTDIKADIATNKADIAKNSARIDSLDKNVANLRKETRQGLAEQAALSGLFQPYNV

• Tag:

  N-terminal 10xHis-tagged

• Type:

  Developed Protein

• Source:

  E.coli

• Field of Research:

  Others

• Relevance:

  Adheres to and induces bacterial uptake by human epithelial cells (Probable) . Upon expression in engineered Y.enterocolitica confers an 11- to 15-fold increase in bacterial adherence and uptake by human epithelial cell lines; part of the uptake is mediated by integrin beta-1 (ITGB1) suggesting it may be a human receptor for NadA. A bacterial cell surface protein; antisera against this protein induce complement-mediated killing of this and other strains.

• Endotoxin:

  Not test

• Purity:

  Greater than 85% as determined by SDS-PAGE.

• Activity:

  Not Test

• Form:

  Liquid or Lyophilized powder

• Buffer:

  If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Molecular Weight:

  35.8 kDa

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

• Protein Length:

  Partial