Recombinant Lactobacillus salivarius Enolase (eno)

• Product Name Alternative:

  (2-phospho-D-glycerate hydro-lyase) (2-phosphoglycerate dehydratase)

• Abbreviation:

  Recombinant Ligilactobacillus salivarius Enolase protein

• Gene Name:

  Eno

• UniProt:

  Q1WSY0

• Expression Region:

  1-441aa

• Organism:

  Ligilactobacillus salivarius (strain UCC118) (Lactobacillus salivarius)

• Target Sequence:

  MSAITEIYAREVLDSRGNPTVEAEVYTQDGGFGRGIVPSGASTGEHEAVELRDGDKDRYMGKGVTKAVANVNDIIAKEIVGYEVTDQVAIDKAMIALDGTPNKGKLGANAILAVSIAVARAAADELQVPLYNYIGGFNAHVLPTPMMNVINGGAHSDNKVDFQEFMIMPVGAPTVKEAIRMGSETFHHLQKLLAADGKVTSVGDEGGFAPDFANNEEPLEYLIKAIEAAGYKPGKDIAIAVDVASSELWDNDSKKYKLRWSTGEEFTTEEFIKYLEGLVAKYPIISIEDPIDENNWDDWVTITSELGKKVQLVGDDFFVTNTEYLRKGIKMGAANSILIKVNQIGTLTETMEAIEMAKEAGYTAIVSHRSGETEDTTIADLVVATNAGQIKTGSMSRTDRLAKYNQLMRIEDQLTDLVAKYKGINSFYNLSEQARQDIINK

• Tag:

  N-terminal 10xHis-tagged and C-terminal Myc-tagged

• Type:

  In Stock Protein

• Source:

  E.coli

• Field of Research:

  Others

• Relevance:

  Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis.

• Endotoxin:

  Not test

• Purity:

  Greater than 85% as determined by SDS-PAGE.

• Activity:

  Not Test

• Form:

  Liquid or Lyophilized powder

• Buffer:

  If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Molecular Weight:

  55.5 kDa

• References & Citations:

  "Multireplicon genome architecture of Lactobacillus salivarius." Claesson M.J., Li Y., Leahy S., Canchaya C., van Pijkeren J.P., Cerdeno-Tarraga A.M., Parkhill J., Flynn S., O'Sullivan G.C., Collins J.K., Higgins D., Shanahan F., Fitzgerald G.F., van Sinderen D., O'Toole P.W. Proc. Natl. Acad. Sci. U.S.A. 103:6718-6723 (2006)

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

• Protein Length:

  Full Length