PNGase F (with His-tag)

• Description:

  PNGase F (Peptide N-Glycosidase F) is a high effective amidase derived from Elizabethkingia miricola . It cleaves asparagine-linked high mannose as well as hybrid and complex oligosaccharides from glycoproteins. The cleavage site of PNGase F is the amide bond between the N-acetylglucosamine (GlcNAc) inside the protein and the asparagine residue, while converting the asparagine residue in the digested protein to aspartic acid. This product is expressed through recombinant expression in E.coli , and it contains a His tag and 50% glycerol. It is commonly used for deglycosylation of antibodies and related proteins.

• Assay Protocol:

  http://en.best-enzymes.com/storage/upload/files/directions/11danbaiyanjiuxilie/EG23303.pdf

• Concentration:

  500 U/μL

• Components:

  • PNGase F (500 U/μl) : 150 μl • 10x Denaturing Buffer: 750 μl • 10x PNGase F Buffer: 1 ml • 10% NP-40: 1 ml

• Shipping Conditions:

  2~8°C

• Storage Conditions:

  -20°C

• Shelf Life:

  2 Years