T4 Polynucleotide Kinase

• Description:

  T4 Polynucleotide Kinase (T4 PNK) is a polyribonucleotide 5'-hydroxyl kinase that catalyzes the transfer of the γ-phosphate group from ATP to the 5'-hydroxyl terminus of oligonucleotide chains, whether they are double-stranded or single-stranded DNA or RNA. Notably, this reaction is reversible. In addition, T4 PNK exhibits 3'-phosphatase activity, which hydrolyzes the 3'-phosphate group from the 3'-phosphate terminus of oligonucleotides, deoxy-3'-monophosphate nucleotides, and deoxy-3'-diphosphate nucleotides.

• Assay Protocol:

  http://en.best-enzymes.com/storage/upload/files/directions/05xiushimei/EG25201.pdf

• Concentration:

  10 U/μL

• Components:

  • T4 Polynucleotide Kinase (10 U/μl) : 50 μl • 10x T4 PNK Buffer: 1 ml

• Shipping Conditions:

  2~8°C

• Storage Conditions:

  -20°C

• Shelf Life:

  2 Years