BsiWI

• Description:

  BsiWI is a Type IIP restriction enzyme that recognizes palindromic sequences. The reaction buffer has been optimized to maximize the functionality of BsiWI. Additionally, the reaction buffer contains recombinant albumin, which enhances the stability of various enzymes.

• Assay Protocol:

  http://en.best-enzymes.com/storage/upload/files/directions/04xianzhimei/EG24506-1.pdf

• Concentration:

  10 U/μL

• Components:

  • BsiWI (10 U/μl) : 30 μl • 10x Cut Buffer C: 1 ml

• Features & Benefits:

  Recommended Reaction Conditions 1x Cut Buffer C ; Incubate at 55°C; Refer to "Protocol for DNA Digestion" for reaction setup. Heat Inactivation Incubation at 80°C for 20 minutes. Methylation sensitivity Cleavage with this restriction enzyme may be blocked or impaired when the substrate DNA is methylated by the CpG methylase.

• Shipping Conditions:

  2~8°C

• Storage Conditions:

  -20°C

• Shelf Life:

  2 Years

• Isoschizomers:

  PspLI, Pfl23II

• Cut Site:

  CGTACG 5'...C ↓ G T A C G...3' 3'...G C A T G ↑ C...5'