BbvCI

• Description:

  BbvCI is derived from the BbvCI gene of Bacillus brevis (L. Ge) and is obtained through recombinant expression in Escherichia coli. BbvCI is a Type IIA restriction enzyme existing as a heterodimer composed of two distinct catalytic subunits, each with an independent cleavage site. It recognizes and cleaves the non-palindromic sequence CCTCAGC (-5/-2), generating 5' cohesive ends with 3-base overhangs. BbvCI uses CutOne® reaction buffer and is compatible with double digestion with other LightNing® restriction enzymes. However, it is advisable to avoid extended digestion (exceeding 3 hours) to prevent star activity.

• Assay Protocol:

  http://en.best-enzymes.com/storage/upload/files/directions/04xianzhimei/25516.pdf

• Concentration:

  2 U/μL

• Components:

  • BbvCI (2 U/μl) : 25 μl • 10x CutOne® Buffer: 1 ml • 10x CutOne® Color Buffer: 1 ml

• Features & Benefits:

  Recommended Reaction Conditions 1x CutOne® Buffer; Incubate at 37°C; Refer to "Protocol for DNA Digestion" for reaction setup. Heat Inactivation Incubation at 80°C for 20 minutes. Methylation sensitivity Cleavage with this restriction enzyme may be blocked or impaired when the substrate DNA is methylated by the CpG methylase.

• Shipping Conditions:

  2~8°C

• Storage Conditions:

  Store at -20°C for 2 years.

• Shelf Life:

  2 Years

• Cut Site:

  CCTCAGC 5'...C C↓T C A G C...3' 3'...G G A G T↑C G ...5'