Phi29 DNA Polymerase

• Description:

  Phi29 DNA Polymerase is a DNA polymerase cloned from Bacillus subtilis phage phi29. phi29 DNA Polymerase exhibits a strong strand-displacement activity, a strong chain affinity, and a single polymerization can achieve a continuous polymerization extension up to 70 kb. In addition, phi29 DNA Polymerase has extremely high fidelity due to its inherent 3'→5' exonuclease activity. However, because of the enzymes's 3'→5' proofreading exonuclease, 3'-modified primers are highly recommended.

• Assay Protocol:

  http://en.best-enzymes.com/storage/upload/files/directions/01juhemei/EG25102.pdf

• Concentration:

  10 U/μL

• Components:

  • phi29 DNA Polymerase (10 U/μl) : 50 μl • 10x phi29 Buffer: 1 ml

• Shipping Conditions:

  2~8°C

• Storage Conditions:

  Store at -20°C for 2 years.

• Shelf Life:

  2 Years