Recombinant Human Calcium release-activated calcium channel protein 1 (ORAI1)

• Product Name Alternative:

  Protein orai-1; Transmembrane protein 142A

• Abbreviation:

  Recombinant Human ORAI1 protein

• Gene Name:

  ORAI1

• UniProt:

  Q96D31

• Expression Region:

  1-301aa

• Organism:

  Homo sapiens (Human)

• Target Sequence:

  MHPEPAPPPSRSSPELPPSGGSTTSGSRRSRRRSGDGEPPGAPPPPPSAVTYPDWIGQSYSEVMSLNEHSMQALSWRKLYLSRAKLKASSRTSALLSGFAMVAMVEVQLDADHDYPPGLLIAFSACTTVLVAVHLFALMISTCILPNIEAVSNVHNLNSVKESPHERMHRHIELAWAFSTVIGTLLFLAEVVLLCWVKFLPLKKQPGQPRPTSKPPASGAAANVSTSGITPGQAAAIASTTIMVPFGLIFIVFAVHFYRSLVSHKTDRQFQELNELAEFARLQDQLDHRGDHPLTPGSHYA

• Tag:

  N-terminal 10xHis-tagged

• Type:

  CF Transmembrane Protein & Developed Protein

• Source:

  In vitro E.coli expression system

• Field of Research:

  Immunology

• Relevance:

  Pore-forming subunit of two major inward rectifying Ca (2+) channels at the plasma membrane: Ca (2+) release-activated Ca (2+) (CRAC) channels and arachidonate-regulated Ca (2+) -selective (ARC) channels (Probable) . Assembles with ORAI2 and ORAI3 to form hexameric CRAC channels that mediate Ca (2+) influx upon depletion of endoplasmic reticulum Ca (2+) store and channel activation by Ca (2+) sensor STIM1, a process known as store-operated Ca (2+) entry (SOCE) . Various pore subunit combinations may account for distinct CRAC channel spatiotemporal and cell-type specific dynamics. ORAI1 mainly contributes to the generation of Ca (2+) plateaus involved in sustained Ca (2+) entry and is dispensable for cytosolic Ca (2+) oscillations, whereas ORAI2 and ORAI3 generate oscillatory patterns. CRAC channels assemble in Ca (2+) signaling microdomains where Ca (2+) influx is coupled to calmodulin and calcineurin signaling and activation of NFAT transcription factors recruited to ORAI1 via AKAP5. Activates NFATC2/NFAT1 and NFATC3/NFAT4-mediated transcriptional responses. CRAC channels are the main pathway for Ca (2+) influx in T cells and promote the immune response to pathogens by activating NFAT-dependent cytokine and chemokine transcription. Assembles with ORAI3 to form channels that mediate store-independent Ca (2+) influx in response to inflammatory metabolites arachidonate or its derivative leukotriene C4, termed ARC and LRC channels respectively. Plays a prominent role in Ca (2+) influx at the basolateral membrane of mammary epithelial cells independently of the Ca (2+) content of endoplasmic reticulum or Golgi stores. May mediate transepithelial transport of large quantities of Ca (2+) for milk secretion

• Endotoxin:

  Not test

• Purity:

  Greater than 95% as determined by SDS-PAGE.

• Activity:

  Not Test

• Form:

  Liquid or Lyophilized powder

• Buffer:

  If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Molecular Weight:

  34.2 kDa

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

• Protein Length:

  Full Length