Recombinant Aquifex aeolicus Reverse gyrase 2 (rgy2), partial

• Product Name Alternative:

  Rgy2

• Abbreviation:

  Recombinant Aquifex aeolicus rgy2 protein, partial

• Gene Name:

  Rgy2

• UniProt:

  O67226

• Expression Region:

  583-1159aa

• Organism:

  Aquifex aeolicus (strain VF5)

• Target Sequence:

  DLFKTTLVIVESPNKARTIAGFFGKPQMRLVEDSVAYEVPLGDRLLVITASLGHVLDLVTDKGFFGVLDDTYPYLPVYDTIKICRETHEQHTEYEYLKKRCKGKIEDKLEIIKGVREVSYEVDEVFIATDPDAEGEKIGYDLYLLSKPFNFNIKRAEFHEVTPKAFREAIQNPREVDLNLVKAQLVRRILDRWVGFTLSHILWDAFGKKWLSAGRVQTPVLGWVIKRYEESKEKKGEILLHVNDFPLKIEIEDLMFAKEIFKDLELADISLSNPQEEEKRPLPPYTTDTVLEDANEKLHLSAHKTMKILQELFEAGYITYHRTDSTRVSDAGKYLVAKPYITKMFGEEYFYPRSWGEGGAHECIRPTRPLEPKDLEFMITAGIAEFEDPENALKVYELIFKRFMASQMRPAKVITEEIILKLPYFEWKERVVTEVKEHGFDLMYPTFKVFPKKEKLEITHKEFREVPKVYPYTQGTLIQEMKRRGLGRPSTYAQIVQTLLERHYVVEEKGFLIPTDLGREVYEYLTKHFPEWTSEELTRKLEEAMDKIERGELDYMEVLKEVHRIKVLLKEEKAFKK

• Tag:

  C-terminal 6xHis-tagged

• Type:

  In Stock Protein

• Source:

  E.coli

• Field of Research:

  Others

• Relevance:

  Modifies the topological state of DNA by introducing positive supercoils in an ATP-dependent process, increasing the linking number in steps of +1. Binds to single-stranded DNA, transiently cleaves and then rejoins the ends, introducing a positive supercoil in the process. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA- (5'-phosphotyrosyl) -enzyme intermediate. Probably involved in rewinding DNA strands in regions of the chromosome that have opened up to allow replication, transcription, DNA repair and/or for DNA protection

• Endotoxin:

  Not test

• Purity:

  Greater than 90% as determined by SDS-PAGE.

• Activity:

  Not Test

• Form:

  Liquid or Lyophilized powder

• Buffer:

  If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Molecular Weight:

  74.5 kDa

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

• Protein Length:

  Partial