Recombinant Severe acute respiratory syndrome coronavirus 2 Nucleoprotein (N) (P13L, Δ31-33, E136D, R203K, G204R, S413R), partial

• Product Name Alternative:

  (N) (Nucleocapsid protein) (NC) (Protein N)

• Abbreviation:

  Recombinant SARS-CoV-2 N protein (P13L, Δ31-33, E136D, R203K, G204R, S413R), partial

• Gene Name:

  N

• UniProt:

  P0DTC9

• Expression Region:

  1-419aa (P13L, Δ31-33, E136D, R203K, G204R, S413R)

• Organism:

  Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2)

• Target Sequence:

  MSDNGPQNQRNAPRITFGGPSDSTGSNQNGGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATDGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSKRTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSRADSTQA

• Tag:

  N-terminal 6xHis-tagged

• Type:

  Developed Protein

• Source:

  E.coli

• Field of Research:

  Microbiology

• Endotoxin:

  Not test

• Purity:

  Greater than 90% as determined by SDS-PAGE.

• Activity:

  Not Test

• Form:

  Liquid or Lyophilized powder

• Buffer:

  If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Molecular Weight:

  49.5 kDa

• References & Citations:

  "Identified a colistin-resistance gene mcr-8.1 in klebsiella pneumoniae." Wang X., Wang Y., Shen Z. Emerg. Microbes Infect. 0:0-0 (2018)

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

• Protein Length:

  Partial