Recombinant Severe acute respiratory syndrome coronavirus 2 Nucleoprotein (N) (I292T) (Active)

• Product Name Alternative:

  /

• Abbreviation:

  Recombinant SARS-CoV-2 N protein (I292T) (Active)

• Gene Name:

  N

• UniProt:

  P0DTC9

• Expression Region:

  1-419aa (I292T)

• Organism:

  Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2)

• Target Sequence:

  MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELTRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA

• Tag:

  N-terminal 6xHis-tagged

• Type:

  Active Protein & In Stock Protein & Coronavirus Protein

• Source:

  E.coli

• Field of Research:

  Microbiology

• Endotoxin:

  Not test

• Purity:

  Greater than 83% as determined by SDS-PAGE.

• Activity:

  Yes

• Bioactivity:

  ①Measured by its binding ability in a functional ELISA. Immobilized N (CSB-EP3325GMY (M10) ) at 2 μg/ml can bind N Mouse Monoclonal Antibody (CSB-MA33255A2m), the EC50 of N Mouse Monoclonal Antibody is 6.560-11.01 ng/ml. ②Measured by its binding ability in a functional ELISA. Immobilized N (CSB-EP3325GMY (M10) ) at 2 μg/ml can bind N Recombinant Antibody (CSB-RA33255A1GMY), the EC50 of N Recombinant Antibody is 3.859-7.358 ng/ml.

• Form:

  Lyophilized powder

• Buffer:

  Lyophilized from 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Molecular Weight:

  49.7 kDa

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

• Protein Length:

  Full Length