Recombinant Human parainfluenza 4b virus Nucleoprotein (N)

• Product Name Alternative:

  Nucleocapsid protein; NP; Protein N

• Abbreviation:

  Recombinant Human parainfluenza 4b virus N protein

• Gene Name:

  N

• UniProt:

  P17241

• Expression Region:

  1-551aa

• Organism:

  Human parainfluenza 4b virus (strain 68-333) (HPIV-4b)

• Target Sequence:

  MSSVLAAYEQFLQTTEDRSFGDQQFVQSDTLKAEIPVFVLNTNDPQQRFTLMNFCLRLAVSSSAKSAIKQGALLSLLSLQATSMQNHLMIAARAPDAALRIIEVDAIDPQDYTLTINPRSGWDDIKIRAYRALSRDLPISLADRTVFVSRDAEHAVCDDMDTYLNRIFSVLIQIWIMVCKCMTAYDQPTGSEERRLAKYKQQGRMLEKYQLQTDARKIIQLVIRESMVIRQFLVQEMLTADKVGAYTNRYYAMVGDIAKYIANVGMSAFFLTLKFGLGNRWKPLALAAFSGELVKLKSFMSLYRRLGDRSRYLALLESPELMEFAPANYPLLFSYAMGVGSVQDPLIRNYQFGRNFLNTSYFQYGVETAMKHQGTVDPKLALELGITDEDRVDIMQSVEKHISGKAGDDISQPAGAFTMSLSRSAFINNNTSQDFSGARLSNYEQGWSGTNQDETRDVYPESTMHRLQNIEPTDSDHNELLMPELESDSNPFNRPRFTVRAPLIPEISHQNPTTRMNRNINTRDNTRADHQDTNEDRGSNVPDDILGDLDN

• Tag:

  N-terminal 10xHis-tagged

• Type:

  In Stock Protein

• Source:

  E.coli

• Field of Research:

  Microbiology

• Relevance:

  Encapsidates the genome, protecting it from nucleases. The nucleocapsid (NC) has a helical structure. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. During replication, encapsidation by N is coupled to RNA synthesis and all replicative products are resistant to nucleases (By similarity) .

• Endotoxin:

  Not test

• Purity:

  Greater than 85% as determined by SDS-PAGE.

• Activity:

  Not Test

• Form:

  Liquid or Lyophilized powder

• Buffer:

  If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Molecular Weight:

  68.5 kDa

• References & Citations:

  "Sequencing analyses and comparison of parainfluenza virus type 4A and 4B NP protein genes." Kondo K., Bando H., Kawano M., Tsurudome M., Komada H., Nishio M., Ito Y. Virology 174:1-8 (1990)

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

• Protein Length:

  Full Length