Recombinant Human C-C chemokine receptor type 1 (CCR1) -VLPs (Active)

• Product Name Alternative:

  C-C chemokine receptor type 1; C-C CKR-1; CC-CKR-1; CCR-1; CCR1; HM145; LD78 receptor; Macrophage inflammatory protein 1-alpha receptor (MIP-1alpha-R) ; RANTES-R; CD191; CCR1; CMKBR1, CMKR1, SCYAR1

• Abbreviation:

  Recombinant Human CCR1 protein-VLPs (Active)

• Gene Name:

  CCR1

• UniProt:

  P32246

• Expression Region:

  1-355aa

• Organism:

  Homo sapiens (Human)

• Target Sequence:

  METPNTTEDYDTTTEFDYGDATPCQKVNERAFGAQLLPPLYSLVFVIGLVGNILVVLVLVQYKRLKNMTSIYLLNLAISDLLFLFTLPFWIDYKLKDDWVFGDAMCKILSGFYYTGLYSEIFFIILLTIDRYLAIVHAVFALRARTVTFGVITSIIIWALAILASMPGLYFSKTQWEFTHHTCSLHFPHESLREWKLFQALKLNLFGLVLPLLVMIICYTGIIKILLRRPNEKKSKAVRLIFVIMIIFFLFWTPYNLTILISVFQDFLFTHECEQSRHLDLAVQVTEVIAYTHCCVNPVIYAFVGERFRKYLRQLFHRRVAVHLVKWLPFLSVDRLERVSSTSPSTGEHELSAGF

• Tag:

  C-terminal 10xHis-tagged (This tag can be tested only under denaturing conditions)

• Type:

  MP-VLP Transmembrane Protein & Active Protein & In Stock Protein

• Source:

  Mammalian cell

• Field of Research:

  Cancer

• Relevance:

  Chemokine receptor that plays a crucial role in regulating immune cell migration, inflammation, and immune responses. Contributes to the inflammatory response by recruiting immune cells, such as monocytes, macrophages, T-cells, and dendritic cells, to sites of inflammation for the clearance of pathogens and the resolution of tissue damage. When activated by its ligands including CCL3, CCL5-9, CCL13-16 and CCL23, triggers a signaling cascade within immune cells, leading to their migration towards the source of the chemokine. For example, mediates neutrophil migration after activation by CCL3 leading to the sequential release of TNF-alpha and leukotriene B4. Also mediates monocyte migration upon CXCL4 binding. Activation by CCL5 results in neuroinflammation through the ERK1/2 signaling pathway.

• Endotoxin:

  Less than 1.0 EU/μg as determined by LAL method.

• Purity:

  The purity information is not available for VLPs proteins.

• Activity:

  Yes

• Bioactivity:

  Measured by its binding ability in a functional ELISA. Immobilized Human CCR1 at 10 μg/mL can bind Anti-CCR1 recombinant antibody (CSB-RA004839MA1HU) . The EC50 is 9.690-10.94 ng/mL. The VLPs (CSB-MP3838) is negative control.

• Form:

  Lyophilized powder

• Buffer:

  Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4.

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein indeionized sterile water to a concentration of 0.1-1.0 mg/mL.Aliquot for long-term storage at -80°C. Solubilize for 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.

• Molecular Weight:

  42.5 kDa

• References & Citations:

  Human LZIP binds to CCR1 and differentially affects the chemotactic activities of CCR1-dependent chemokines. Ko J., Jang S.W., Kim Y.S., Kim I.S., Sung H.J., Kim H.-H., Park J.Y., Lee Y.H., Kim J., Na D.S. FASEB J. 18:890-892 (2004)

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

• Protein Length:

  Full Length