Recombinant Streptococcus mutans serotype c UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-lysine ligase (murE)

• Product Name Alternative:

  L-lysine-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:L-Lys ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase

• Abbreviation:

  Recombinant Streptococcus mutans serotype c murE protein

• Gene Name:

  MurE

• UniProt:

  Q8DST2

• Expression Region:

  1-483aa

• Organism:

  Streptococcus mutans serotype c (strain ATCC 700610 / UA159)

• Target Sequence:

  MITIETVLTILKNNANFREIIDGKHYQYKYSNPEVAFHHISYDSRDIKASTLFFVKGATFKKEFLEKAIESGLTFYVAEKDYQVGIPLILVNNIKNAMCLIAKEFYDNPQDKLKTLAFTGTKGKTTAAYFAYHILKQGYRPAMISTMNTTLDGKTFFKSKLTTPESLDLFKMMAQAVANGMTHLIMEVSSQAYLVGRVYGLTFDVGVFLNISPDHIGPIEHPTFEDYFYHKRLLLKHSRYVIVNSQMNHFALIKEQVADQPHDFYGKYSDNQIINSKAFSFDLTGKLAGHYDIQLTGSFNQENAVAAALACLQLGASQTDIQKGIVQTTVPGRMEVLIQKNGAKVFVDYAHNGDSLEKLLSVVEEHQKGTLILILGAPGNKGESRRADFGYVINAHPELQVILTADDPNNEDPQLISQEIAHHIKRPVNIIVDRKQAIQKAMSLTQSENDAVIIAGKGADAFQIIKGKRTAYAGDIEVARKYL

• Tag:

  C-terminal 6xHis-tagged

• Type:

  Developed Protein

• Source:

  E.coli

• Field of Research:

  Others

• Relevance:

  Catalyzes the addition of L-lysine to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan

• Endotoxin:

  Not test

• Purity:

  Greater than 90% as determined by SDS-PAGE.

• Activity:

  Not Test

• Form:

  Liquid or Lyophilized powder

• Buffer:

  If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Molecular Weight:

  60.9 kDa

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

• Protein Length:

  Full Length