Recombinant Drosophila melanogaster Nuclear RNA export factor 2 (nxf2), partial

• Abbreviation:

  Recombinant Drosophila melanogaster nxf2 protein, partial

• Gene Name:

  Nxf2

• UniProt:

  Q9VV73

• Expression Region:

  573-841aa

• Organism:

  Drosophila melanogaster (Fruit fly)

• Target Sequence:

  QKNFLCDTGAYELVGAFLENYLREFENDEFRHNLYKYYSENSIFTLTCNYNVVQNHQTPKILQRLSKYNRHARNLRNKDYSKASDGVFFGCTYIVEILLQLPRVTHDFHSLQTDVMHYNGKGAVIYVAGLLRDEPPSTRNGHGSKTDIGGVLLGFSRQFVVTFDEANLGLGKRARRLKIANERLHITNPSKTAIRNAFSVNFPDPSERQAEEDSLDVKDHKLLLFQEVTGLISTWVTSIVEEADWDFERALKLFIQKNADHEIPDLAFA

• Tag:

  C-terminal 6xHis-tagged

• Type:

  In Stock Protein

• Source:

  Baculovirus

• Field of Research:

  Others

• Relevance:

  May be involved in the export of mRNA from the nucleus to the cytoplasm. In the ovaries, forms a complex with nxf2, piwi and Nxt1 which acts as effectors of cotranscriptional transposon silencing. On recruitment to a target transcript, interacts with single stranded RNA, thereby anchoring the complex via the nascent target transcript to chromatin and allowing Panx to recruit silencing effectors to establishing repressive heterochromatin at transposon loci. Does not affect piRNA biogenesis. The interaction with Panx stabilizes the nuclear protein complex. Does not bind nucleoporins, but regulates sbr/Nxf1 binding to nucleoporins and, indirectly, transposon exports.

• Endotoxin:

  Not test

• Purity:

  Greater than 85% as determined by SDS-PAGE.

• Activity:

  Not Test

• Form:

  Liquid or Lyophilized powder

• Buffer:

  If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Molecular Weight:

  33.7 kDa

• References & Citations:

  "NXF1/p15 heterodimers are essential for mRNA nuclear export in Drosophila." Herold A., Klymenko T., Izaurralde E. RNA 7:1768-1780 (2001)

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

• Protein Length:

  Partial