Recombinant Human Excitatory amino acid transporter 1 (SLC1A3), partial

• Product Name Alternative:

  (Sodium-dependent glutamate/aspartate transporter 1) (GLAST-1) (Solute carrier family 1 member 3)

• Abbreviation:

  Recombinant Human SLC1A3 protein, partial

• Gene Name:

  SLC1A3

• UniProt:

  P43003

• Expression Region:

  69-86aa

• Organism:

  Homo sapiens (Human)

• Target Sequence:

  RPYRMSYREVKYFSFPGE

• Tag:

  N-terminal 6xHis-KSI-tagged

• Type:

  Developed Protein

• Source:

  E.coli

• Field of Research:

  Neuroscience

• Relevance:

  Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate . Functions as a symporter that transports one amino acid molecule together with two or three Na (+) ions and one proton, in parallel with the counter-transport of one K (+) ion . Mediates Cl (-) flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na (+) symport . Plays a redundant role in the rapid removal of released glutamate from the synaptic cleft, which is essential for terminating the postsynaptic action of glutamate .

• Endotoxin:

  Not test

• Purity:

  Greater than 85% as determined by SDS-PAGE.

• Activity:

  Not Test

• Form:

  Liquid or Lyophilized powder

• Buffer:

  If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Molecular Weight:

  17.6 kDa

• References & Citations:

  "The amino acid transporter, SLC1A3, is plasma membrane-localised in adipocytes and its activity is insensitive to insulin." Krycer J.R., Fazakerley D.J., Cater R.J., C Thomas K., Naghiloo S., Burchfield J.G., Humphrey S.J., Vandenberg R.J., Ryan R.M., James D.E. FEBS Lett. 591:322-330 (2017)

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

• Protein Length:

  Partial