Immobilized Papain

• Target:

  Immobilized Papain

• Nature:

  Native

• Type:

  Native Protein

• Applications:

  FuncS

• Field of Research:

  Cell Biology

• Relevance:

  Papain is a nonspecific, cysteine-endopeptidase isolated from papaya latex that is used in a wide variety of applications. When IgG molecules are incubated with papain in the presence of cysteine, one or more peptide bonds in the hinge region are split, producing three fragments of similar size: two Fab fragments and one Fc fragment. The Fc fragments can be separated from the Fab fragments using immobilized Protein A or by ion exchange chromatography.

• Purity:

  >95% (SDS-PAGE)

• Bioactivity:

  Activity: 2000 units per milligram of papain before immobilization Loading: 10mg papain/g of settled gel

• Form:

  Liquid

• Storage Conditions:

  Aliquot and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles.

• Protein Length:

  Full length protein