Recombinant Human C-C chemokine receptor type 4 (CCR4) (Active)

• Product Name Alternative:

  C-C chemokine receptor type 4; C-C CKR-4; CC-CKR-4; CCR-4; CCR4; K5-5; CD194

• Abbreviation:

  Recombinant Human CCR4 protein (Active)

• Gene Name:

  CCR4

• UniProt:

  P51679

• Expression Region:

  1-360aa

• Organism:

  Homo sapiens (Human)

• Target Sequence:

  MNPTDIADTTLDESIYSNYYLYESIPKPCTKEGIKAFGELFLPPLYSLVFVFGLLGNSVVVLVLFKYKRLRSMTDVYLLNLAISDLLFVFSLPFWGYYAADQWVFGLGLCKMISWMYLVGFYSGIFFVMLMSIDRYLAIVHAVFSLRARTLTYGVITSLATWSVAVFASLPGFLFSTCYTERNHTYCKTKYSLNSTTWKVLSSLEINILGLVIPLGIMLFCYSMIIRTLQHCKNEKKNKAVKMIFAVVVLFLGFWTPYNIVLFLETLVELEVLQDCTFERYLDYAIQATETLAFVHCCLNPIIYFFLGEKFRKYILQLFKTCRGLFVLCQYCGLLQIYSADTPSSSYTQSTMDHDLHDAL

• Tag:

  N-terminal 10xHis-tagged

• Type:

  CF Transmembrane Protein & Active Protein & In Stock Protein

• Source:

  In vitro E.coli expression system

• Field of Research:

  Others

• Relevance:

  High affinity receptor for the C-C type chemokines CCL17/TARC, CCL22/MDC and CKLF isoform 1/CKLF1. The activity of this receptor is mediated by G (i) proteins which activate a phosphatidylinositol-calcium second messenger system. Can function as a chemoattractant homing receptor on circulating memory lymphocytes and as a coreceptor for some primary HIV-2 isolates. In the CNS, could mediate hippocampal-neuron survival.

• Endotoxin:

  Not test

• Purity:

  Greater than 85% as determined by SDS-PAGE.

• Activity:

  Yes

• Bioactivity:

  ①Measured by its binding ability in a functional ELISA. Immobilized Human CCR4 at 5 μg/mL can bind Anti-CCR4 recombinant antibody (CSB-RA004843MA01HU) . The EC50 is 2.803-3.369 ng/mL. ②Human CCR4 Full Length Protein captured on CM5 chip can bind Human CCR4 Monoclonal Antibody (CSB-RA004843MA01HU) with an affinity constant of 3.16 nM as detected by SPR Assay (Biacore T200) . ③Human CCR4 Monoclonal Antibody (CSB-RA004843MA01HU) captured on Protein A Chip can bind Human CCR4 Full Length Protein with an affinity constant of 1.71 nM as detected by MetaSPR Assay (in presence of FOS-12) (WeSPRTM200) .

• Form:

  Lyophilized powder

• Buffer:

  Lyophilized from PBS, 0.05% FOS-12, 6% Trehalose, pH 8.0.

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Function:

  High affinity receptor for the C-C type chemokines CCL17/TARC, CCL22/MDC and CKLF isoform 1/CKLF1. The activity of this receptor is mediated by G (i) proteins which activate a phosphatidylinositol-calcium second messenger system. Can function as a chemoattractant homing receptor on circulating memory lymphocytes and as a coreceptor for some primary HIV-2 isolates. In the CNS, could mediate hippocampal-neuron survival.

• Molecular Weight:

  44.2 kDa

• References & Citations:

  Chemokine-like factor 1 is a functional ligand for CC chemokine receptor 4 (CCR4) . Wang Y., Zhang Y., Yang X., Han W., Liu Y., Xu Q., Zhao R., Di C., Song Q., Ma D. Life Sci. 78:614-621 (2006)

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

• Protein Length:

  Full Length