Recombinant Paraponera clavata Delta-paraponeritoxin-Pc1a

• Product Name Alternative:

  Pac-TX

• Abbreviation:

  Recombinant Paraponera clavata Delta-paraponeritoxin-Pc1a protein

• UniProt:

  P41736

• Expression Region:

  1-25aa

• Organism:

  Paraponera clavata (Bullet ant) (Izuela)

• Target Sequence:

  FLPLLILGSLLMTPPVIQAIHDAQR

• Tag:

  N-terminal 6xHis-tagged

• Type:

  Developed Protein

• Source:

  Baculovirus

• Field of Research:

  Others

• Relevance:

  Blocks nicotinic synaptic transmission in insect CNS and affects voltage-gated sodium channels (Nav) . It is a strong, but very slowly acting agonist for smooth muscles and its blocks synaptic transmission in the insect CNS in a concentration-dependent manner and depolarizes giant interneurons. Also affects neuromuscular transmission in rat tissue.

• Endotoxin:

  Not test

• Purity:

  Greater than 90% as determined by SDS-PAGE.

• Activity:

  Not Test

• Form:

  Liquid or Lyophilized powder

• Buffer:

  If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Function:

  Blocks nicotinic synaptic transmission in insect CNS and affects voltage-gated sodium channels (Nav) . It is a strong, but very slowly acting agonist for smooth muscles and its blocks synaptic transmission in the insect CNS in a concentration-dependent manner and depolarizes giant interneurons. Also affects neuromuscular transmission in rat tissue.

• Molecular Weight:

  3.7 kDa

• References & Citations:

  "Pharmacological characterization and chemical fractionation of the venom of the ponerine ant, Paraponera clavata (F.) ." Piek T., Hue B., Mantel P., Nakajima T., Schmidt J.O. Comp. Biochem. Physiol. 99C:481-486 (1991)

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

• Protein Length:

  Full Length