Recombinant Eisenia fetida Lysenin-related protein 2

• Product Name Alternative:

  Fetidin (Hemolysin) (Lysenin-3) (efL3) (LRP-2)

• Abbreviation:

  Recombinant Eisenia fetida Lysenin-related protein 2 protein

• UniProt:

  O18425

• Expression Region:

  1-300aa

• Organism:

  Eisenia fetida (Red wiggler worm)

• Target Sequence:

  MSSRAGIAEGYEQIEVDVVAVWKEGYVYENRGSTSVEQKIKITKGMRNLNSETKTLTASHSIGSTISTGDLFEIATVDVSYSYSHEESQVSMTETEVYESKEIEHTITIPPTSKFTRWQLNADVGGADIEYMYLIDEVTPIGGTLSIPQVIKSRAKILVGREIYLGETEIRIKHADRKEYMTVVSRKSWPAATLGHSKLYKFVLYEDMYGFRIKTLNTMYSGYEYAYSSDQGGIYFDQGSDNPKQRWAINKSLPLRHGDVVTFMNKYFTRSGLCYYDGPATDVYCLDKREDKWILEVVKP

• Tag:

  N-terminal 10xHis-tagged and C-terminal Myc-tagged

• Type:

  In Stock Protein

• Source:

  E.coli

• Field of Research:

  Others

• Relevance:

  Pore-forming toxin that specifically binds sphingomyelin in the plasma membrane of various cells. Has hemolytic activity. It also has antibacterial activities against B.megaterium.

• Endotoxin:

  Not test

• Purity:

  Greater than 85% as determined by SDS-PAGE.

• Activity:

  Not Test

• Form:

  Liquid or Lyophilized powder

• Buffer:

  If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Function:

  Pore-forming toxin that specifically binds sphingomyelin in the plasma membrane of various cells (By similarity) . Has hemolytic activity. It also has antibacterial activities against B.megaterium.

• Molecular Weight:

  41.6 kDa

• References & Citations:

  "Relationship between hemolytic molecules in Eisenia fetida earthworms." Prochazkova P., Silerova M., Felsberg J., Joskova R., Beschin A., De Baetselier P., Bilej M. Dev. Comp. Immunol. 30:381-392 (2006)

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

• Protein Length:

  Full Length