Recombinant Aspergillus flavus Exochitosanase, partial

• Product Name Alternative:

  Exo-beta-D-glucosaminidase

• Abbreviation:

  Recombinant Aspergillus flavus Exochitosanase protein, partial

• UniProt:

  P83488

• Expression Region:

  1-17aa

• Organism:

  Aspergillus flavus

• Target Sequence:

  LPTGPNNPTTLDNSSII

• Tag:

  N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged

• Type:

  Developed Protein

• Source:

  E.coli

• Field of Research:

  Others

• Relevance:

  Exohydrolysis of beta-1,4-linkages between N-acetyl-D-glucosamine and D-glucosamine residues, and D-glucosamine and D-glucosamine residues in chitosan.

• Endotoxin:

  Not test

• Purity:

  Greater than 85% as determined by SDS-PAGE.

• Activity:

  Not Test

• Form:

  Liquid or Lyophilized powder

• Buffer:

  If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Function:

  Exohydrolysis of beta-1,4-linkages between N-acetyl-D-glucosamine and D-glucosamine residues, and D-glucosamine and D-glucosamine residues in chitosan.

• Molecular Weight:

  17.8 kDa

• References & Citations:

  "Purification and characterization of the exo-beta-D-glucosaminidase from the fungus, A. flavus IAM2044." Ji J.-H., Hur J.-W. J. Microbiol. Biotechnol. 13:269-275 (2003)

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

• Protein Length:

  Partial