Recombinant Staphylococcus aureus Phenol-soluble modulin alpha 1 peptide (psmA1)

• Product Name Alternative:

  PsmA1; MW0406.4; Phenol-soluble modulin alpha 1 peptide

• Abbreviation:

  Recombinant Staphylococcus aureus psmA1 protein

• Gene Name:

  PsmA1

• UniProt:

  A9JX05

• Expression Region:

  1-21aa

• Organism:

  Staphylococcus aureus (strain MW2)

• Target Sequence:

  MGIIAGIIKVIKSLIEQFTGK

• Tag:

  N-terminal 6xHis-sumostar-tagged

• Type:

  Developed Protein

• Source:

  Yeast

• Field of Research:

  Cell Biology

• Relevance:

  Peptide which can recruit, activate and subsequently lyse human neutrophils, thus eliminating the main cellular defense against infection. Stimulates the secretion of the chemotactic factor interleukin-8. The ensuing activation process triggers an inflammatory response in the host, thus contributing greatly to virulence. Also possesses hemolytic activity, which may contribute to the development of disease.

• Endotoxin:

  Not test

• Purity:

  Greater than 85% as determined by SDS-PAGE.

• Activity:

  Not Test

• Form:

  Liquid or Lyophilized powder

• Buffer:

  If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Function:

  Peptide which can recruit, activate and subsequently lyse human neutrophils, thus eliminating the main cellular defense against infection. Stimulates the secretion of the chemotactic factor interleukin-8 (IL-8) . The ensuing activation process triggers an inflammatory response in the host, thus contributing greatly to virulence. Also possesses hemolytic activity, which may contribute to the development of disease.

• Molecular Weight:

  24.3 kDa

• References & Citations:

  "Identification of novel cytolytic peptides as key virulence determinants for community-associated MRSA." Wang R., Braughton K.R., Kretschmer D., Bach T.-H.L., Queck S.Y., Li M., Kennedy A.D., Dorward D.W., Klebanoff S.J., Peschel A., DeLeo F.R., Otto M. Nat. Med. 13:1510-1514 (2007)

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

• Protein Length:

  Full Length