Recombinant Turnip mosaic virus Polyprotein, partial

• Product Name Alternative:

  /

• Abbreviation:

  Recombinant Turnip mosaic virus polyprotein, partial

• UniProt:

  Q9ICI2

• Expression Region:

  2360-2876aa

• Organism:

  Turnip mosaic virus (strain Japanese) (TuMV)

• Target Sequence:

  TQQNRWMFEQLNGNLKAIAHCPSQLVTKHTVKGKCQMFDLYLKLHDEAREYFQPMLGQYQKSKLNREAYAKDLLKYATPIEAGNIDCDLFEKTVEIVVSDLRGYGFETCNYVTDENDIFEALNMKSAVGALYKGKKKDYFAEFTPEMKEEILKQSCERLFLGKMGVWNGSLKAELRPLEKVEANKTRTFTAAPLDTLLGGKVCVDDFNNQFYDHNLRAPWSVGMTKFYCGWDRLLESLPDGWVYCDADGSQFDSSLSPYLINAVLNIRLGFMEEWDIGEVMLRNLYTEIVYTPISTPDGTLVKKFKGNNSGQPSTVVDNTLMVILAVNYSLKKSGIPSELRDSIIRFFVNGDDLLLSVHPEYEYILDTMADNFRELGLKYTFDSRTREKGDLWFMSHQGHKREGIWIPKLEPERIVSILEWDRSKEPCHRLEAICAAMIESWGYDKLTHEIRKFYAWMIEQAPFSSLAQEGKAPYIAETALRKLYLDKEPAQEDLTHYLQAIFEDYEDGAEACVYHQ

• Tag:

  N-terminal 10xHis-tagged and C-terminal Myc-tagged

• Type:

  In Stock Protein

• Source:

  E.coli

• Field of Research:

  Others

• Relevance:

  Helper component proteinase: required for aphid transmission and also has proteolytic activity. Only cleaves a Gly-Gly dipeptide at its own C-terminus. Interacts with virions and aphid stylets. Acts as a suppressor of RNA-mediated gene silencing, also known as post-transcriptional gene silencing (PTGS), a mechanism of plant viral defense that limits the accumulation of viral RNAs. May have RNA-binding activity.

• Endotoxin:

  Not test

• Purity:

  Greater than 85% as determined by SDS-PAGE.

• Activity:

  Not Test

• Form:

  Liquid or Lyophilized powder

• Buffer:

  If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Molecular Weight:

  66.6 kDa

• References & Citations:

  "The cylindrical inclusion gene of Turnip mosaic virus encodes a pathogenic determinant to the Brassica resistance gene TuRB01." Jenner C.E., Sanchez F., Nettleship S.B., Foster G.D., Ponz F., Walsh J.A. Mol. Plant Microbe Interact. 13:1102-1108 (2000)

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

• Protein Length:

  Partial