Recombinant Arabidopsis thaliana UDP-glycosyltransferase 89C1 (UGT89C1)

• Product Name Alternative:

  Flavonol 7-O-rhamnosyltransferase UDP-rhamnose: flavonol 7-O-rhamnosyltransferase

• Abbreviation:

  Recombinant Mouse-ear cress UGT89C1 protein

• Gene Name:

  UGT89C1

• UniProt:

  Q9LNE6

• Expression Region:

  1-435aa

• Organism:

  Arabidopsis thaliana (Mouse-ear cress)

• Target Sequence:

  MTTTTTKKPHVLVIPFPQSGHMVPHLDLTHQILLRGATVTVLVTPKNSSYLDALRSLHSPEHFKTLILPFPSHPCIPSGVESLQQLPLEAIVHMFDALSRLHDPLVDFLSRQPPSDLPDAILGSSFLSPWINKVADAFSIKSISFLPINAHSISVMWAQEDRSFFNDLETATTESYGLVINSFYDLEPEFVETVKTRFLNHHRIWTVGPLLPFKAGVDRGGQSSIPPAKVSAWLDSCPEDNSVVYVGFGSQIRLTAEQTAALAAALEKSSVRFIWAVRDAAKKVNSSDNSVEEDVIPAGFEERVKEKGLVIRGWAPQTMILEHRAVGSYLTHLGWGSVLEGMVGGVMLLAWPMQADHFFNTTLIVDKLRAAVRVGENRDSVPDSDKLARILAESAREDLPERVTLMKLREKAMEAIKEGGSSYKNLDELVAEMCL

• Tag:

  N-terminal 10xHis-tagged

• Type:

  In Stock Protein

• Source:

  Yeast

• Field of Research:

  Others

• Relevance:

  Flavonol 7-O-rhamnosyltransferase that acts on kaempferol 3-O-glucoside and other 3-O-glycosylated flavonols like quercetin 3-O-glucoside, quercetin 3-O-galactoside and isorhamnetin 3-O-glucoside. Shows a strict specificity for UDP-rhamnose as donor and does not act on flavonol aglycones or 3-O-glycosylated anthocyanins.

• Endotoxin:

  Not test

• Purity:

  Greater than 85% as determined by SDS-PAGE.

• Activity:

  Not Test

• Form:

  Liquid or Lyophilized powder

• Buffer:

  If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Function:

  Flavonol 7-O-rhamnosyltransferase that acts on kaempferol 3-O-glucoside and other 3-O-glycosylated flavonols like quercetin 3-O-glucoside, quercetin 3-O-galactoside and isorhamnetin 3-O-glucoside. Shows a strict specificity for UDP-rhamnose as donor and does not act on flavonol aglycones or 3-O-glycosylated anthocyanins.

• Molecular Weight:

  50.6 kDa

• References & Citations:

  "Phylogenetic analysis of the UDP-glycosyltransferase multigene family of Arabidopsis thaliana." Li Y., Baldauf S., Lim E.K., Bowles D.J. J. Biol. Chem. 276:4338-4343 (2001)

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

• Protein Length:

  Full length