Recombinant Escherichia coli Oxidoreductase YdhF (ydhF)

• Product Name Alternative:

  YdhF; b1647; JW1639; Oxidoreductase YdhF; EC 1.-.-.-

• Abbreviation:

  Recombinant E.coli ydhF protein

• Gene Name:

  YdhF

• UniProt:

  P76187

• Expression Region:

  1-298aa

• Organism:

  Escherichia coli (strain K12)

• Target Sequence:

  MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDLGVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITDRDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNFTPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLFNDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAETLKMTRQQWFRIRKAALGYDVP

• Tag:

  N-terminal 6xHis-tagged

• Type:

  Developed Protein

• Source:

  E.coli

• Field of Research:

  Others

• Relevance:

  May function as oxidoreductase.

• Endotoxin:

  Not test

• Purity:

  Greater than 90% as determined by SDS-PAGE.

• Activity:

  Not Test

• Form:

  Liquid or Lyophilized powder

• Buffer:

  If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Function:

  May function as oxidoreductase.

• Molecular Weight:

  37.7 kDa

• References & Citations:

  "Structural genomics of highly conserved microbial genes of unknown function in search of new antibacterial targets." Abergel C., Coutard B., Byrne D., Chenivesse S., Claude J.-B., Deregnaucourt C., Fricaux T., Gianesini-Boutreux C., Jeudy S., Lebrun R., Maza C., Notredame C., Poirot O., Suhre K., Varagnol M., Claverie J.-M. J. Struct. Funct. Genomics 4:141-157 (2003)

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

• Protein Length:

  Full Length