Recombinant Cryptococcus neoformans var. neoformans serotype D Orotate phosphoribosyltransferase (URA5)

• Product Name Alternative:

  URA5; CNG03730; Orotate phosphoribosyltransferase; OPRT; OPRTase; EC 2.4.2.10

• Abbreviation:

  Recombinant Cryptococcus neoformans var. neoformans serotype D URA5 protein

• Gene Name:

  URA5

• UniProt:

  P0CS95

• Expression Region:

  1-225aa

• Organism:

  Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) (Filobasidiella neoformans)

• Target Sequence:

  MSSQALDSAKVAFIEAAIEHGVLLFGNFTLKSGRQSPYFFNAGLLYSSSLLSTTAQAYAKVLSSSRIPDFDVLFGPAYKGISLAAVSAVSLYQQTGKDIGYCYNRKEKKDHGEGGTMVGAPLKGRIVIIDDVLTSGKAIREAIDILKASPEAKLVGIVQLVDRQEKGQSGSGKSTVQEVEEEFGVPVEPIIGLDDIVKYLESSGKWEKELQEVRKYRAEYGVQRS

• Tag:

  N-terminal 6xHis-sumostar-tagged

• Type:

  Developed Protein

• Source:

  Yeast

• Field of Research:

  Others

• Relevance:

  Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) .

• Endotoxin:

  Not test

• Purity:

  Greater than 85% as determined by SDS-PAGE.

• Activity:

  Not Test

• Form:

  Liquid or Lyophilized powder

• Buffer:

  If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Function:

  Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) .

• Molecular Weight:

  40.4 kDa

• References & Citations:

  "Isolation of the URA5 gene from Cryptococcus neoformans var. neoformans and its use as a selective marker for transformation." Edman J.C., Kwon-Chung K.J. Mol. Cell. Biol. 10:4538-4544 (1990)

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

• Protein Length:

  Full Length