Recombinant Xenopus laevis GATA-binding factor 1-A (gata1-a)

• Product Name Alternative:

  Transcription factor xGATA-1A

• Abbreviation:

  Recombinant Xenopus laevis gata1-a protein

• Gene Name:

  Gata1-a

• UniProt:

  P23767

• Expression Region:

  1-359aa

• Organism:

  Xenopus laevis (African clawed frog)

• Target Sequence:

  MDYTTLTTQDPDPNYTESGLASTSEDSQFLYGLGGESSPGHYGGAVSSRAVGGFRHSPVFQTFPLHWPETSAGIPSNLTAYGRSTGTLSFYPSAASALGPITSPPLYSASSFLLGSAPPAEREGSPKFLETLKTERASPLTSDLLPLEPRSPSILQVGYIGGGGQEFSLFQSTEDRECVNCGATVTPLWRRDMSGHYLCNACGLYHKMNGQNRPLIRPKKRLIVSKRAGTQCSNCHTSTTTLWRRNASGDPVCNACGLYYKLHNVNRPLTMKKEGIQTRNRKVSSRSKKKKQLDNPFEPPKAGVEEPSPYPFGPLLFHGQMPPMGHMINPPHHFLQSPRISHSAPAVSYRQAASGVTPP

• Tag:

  N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged

• Type:

  Developed Protein

• Source:

  E.coli

• Field of Research:

  Epigenetics and Nuclear Signaling

• Relevance:

  Transcription factor that acts synergistically with tal1/scl and lmo2 to specify embryonic dorsal mesoderm to a hematopoietic fate.

• Endotoxin:

  Not test

• Purity:

  Greater than 90% as determined by SDS-PAGE.

• Activity:

  Not Test

• Form:

  Liquid or Lyophilized powder

• Buffer:

  If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Function:

  Transcription factor that acts synergistically with tal1/scl and lmo2 to specify embryonic dorsal mesoderm to a hematopoietic fate.

• Molecular Weight:

  59 kDa

• References & Citations:

  "Expression of GATA-binding proteins during embryonic development in Xenopus laevis." Zon L.I., Mather C., Burgess S., Bolce M.E., Harland R.M., Orkin S.H. Proc. Natl. Acad. Sci. U.S.A. 88:10642-10646 (1991)

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

• Protein Length:

  Full Length