Recombinant Geobacillus stearothermophilus Glycerol-1-phosphate dehydrogenase [NAD (P) +] (egsA)

• Product Name Alternative:

  Enantiomeric glycerophosphate synthase sn-glycerol-1-phosphate dehydrogenase

• Abbreviation:

  Recombinant Geobacillus stearothermophilus egsA protein

• Gene Name:

  EgsA

• UniProt:

  B3EYN8

• Expression Region:

  1-404aa

• Organism:

  Geobacillus stearothermophilus (Bacillus stearothermophilus)

• Target Sequence:

  MNQTLEEVVSLARQCGCGHRHYDIPIEQMVVGREAFARLVAYLRHKRYERVAIVADDHTFAAVERSLCDQLENGSIRYTVCLVQPDENGDVIADERSIVQVLLETPDDVDVLIAVGAGTIHDITRFSSYKMRIPFISVPTAPSVDGFTSMGAPLIIRGVKKTIQAQAPIAVFAHTGVLCQSPKEMIAAGFGDMVAKYTSLADWQFAHWMADEPYCPFVHQLTEQSLQTCVDHIDDIAAGGEQGIRVLMDALLQSGIAMLLMGQSYSASGAEHHLSHYWEMEFLRQKKRQVLHGAKVGVSTPIIIEHYQRVFWPLLNELEKRPKSMDEATWERLKANTASIRELLESLPSPERIRTMLAKVGGAIAPEQLGIDPQLVERSLREAHRLRLNRFTMLYFLNELIFVE

• Tag:

  N-terminal 6xHis-tagged

• Type:

  Developed Protein

• Source:

  Yeast

• Field of Research:

  Others

• Relevance:

  Catalyzes the NAD (P) H-dependent reduction of dihydroxyacetonephosphate (DHAP or glycerone phosphate) to glycerol 1-phosphate (G1P) . The G1P thus generated is probably used for the synthesis of phosphoglycerolipids in Gram-positive bacterial species.

• Endotoxin:

  Not test

• Purity:

  Greater than 90% as determined by SDS-PAGE.

• Activity:

  Not Test

• Form:

  Liquid or Lyophilized powder

• Buffer:

  If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Function:

  Catalyzes the NAD (P) H-dependent reduction of dihydroxyacetonephosphate (DHAP or glycerone phosphate) to glycerol 1-phosphate (G1P) . The G1P thus generated is probably used for the synthesis of phosphoglycerolipids in Gram-positive bacterial species.

• Molecular Weight:

  47.4 kDa

• References & Citations:

  "Hemicellulose utilization cluster in Geobacillus stearothermophilus strain T-6."Shoham Y., Shulami S., Ben-David A., Langut Y. Submitted (JUN-2008)

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

• Protein Length:

  Full Length