Recombinant Giardia lamblia virus Major capsid protein (gag), partial

• Product Name Alternative:

  Gag protein Major coat protein

• Abbreviation:

  Recombinant Giardia lamblia virus Major capsid protein, partial

• Gene Name:

  Gag

• UniProt:

  Q67652

• Expression Region:

  1-206aa

• Organism:

  Giardia lamblia virus (isolate Wang) (GLV)

• Target Sequence:

  MVWGTGYGNPSPLNPYGFASLHRGGLNPLILAPENITFDTLNTHNDHEETHGESPEVPKASIAPAGRQNVPVLQQNQENEDNHALGGSEDAKDEREIRFSAIKTLYNYYSEGPSTPIMPHLVNRLRGLDALAKIDATLTKVDMNAAYIFALRPTFPYSYGYKQRFSNRRLTTSALCYARTGLSSFLTVDKTYTSNSPLKGGSRGWP

• Tag:

  N-terminal 6xHis-tagged

• Type:

  In Stock Protein

• Source:

  E.coli

• Field of Research:

  Microbiology

• Relevance:

  Capsid protein self-assembles to form an icosahedral capsid with a T=2 symmetry, 35 nm in diameter, and consisting of 60 capsid proteins asymmetric dimers. The capsid encapsulates the genomic dsRNA and the polymerase and remains intact following cell entry to protect the dsRNA from degradation and to prevent unfavorable antiviral responses in the host cell during all the replication cycle of the virus. Nacent transcripts are transcribed within the structural confines of the virion and are extruded into the cytoplasm (By similarity) .

• Endotoxin:

  Not test

• Purity:

  Greater than 90% as determined by SDS-PAGE.

• Activity:

  Not Test

• Form:

  Liquid or Lyophilized powder

• Buffer:

  If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Function:

  Capsid protein self-assembles to form an icosahedral capsid with a T=2 symmetry, 35 nm in diameter, and consisting of 60 capsid proteins asymmetric dimers. The capsid encapsulates the genomic dsRNA and the polymerase and remains intact following cell entry to protect the dsRNA from degradation and to prevent unfavorable antiviral responses in the host cell during all the replication cycle of the virus. Nascent transcripts are transcribed within the structural confines of the virion and are extruded into the cytoplasm (By similarity) .

• Molecular Weight:

  26.8 kDa

• References & Citations:

  "Giardiavirus double-stranded RNA genome encodes a capsid polypeptide and a gag-pol-like fusion protein by a translation frameshift."Wang A.L., Yang H.M., Shen K.A., Wang C.C.Proc. Natl. Acad. Sci. U.S.A. 90:8595-8599 (1993) .

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

• Protein Length:

  Partial