Recombinant Escherichia coli Lipid kinase YegS (yegS)

• Product Name Alternative:

  YegS; b2086; JW2070; Lipid kinase YegS; EC 2.7.1.-

• Abbreviation:

  Recombinant E.coli yegS protein

• Gene Name:

  YegS

• UniProt:

  P76407

• Expression Region:

  1-299aa

• Organism:

  Escherichia coli (strain K12)

• Target Sequence:

  MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKGDAARYVEEARKFGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATSVGIPEALDKALKLAIAGDAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALGSVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALINDGLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHDITFNLDGEPLSGQNFHIEILPAALRCRLPPDCPLLR

• Tag:

  N-terminal 6xHis-SUMO-tagged

• Type:

  Developed Protein

• Source:

  E.coli

• Field of Research:

  Microbiology

• Relevance:

  In vitro phosphorylates phosphatidylglycerol but not diacylglycerol; the in vivo substrate is unknown.

• Endotoxin:

  Not test

• Purity:

  Greater than 90% as determined by SDS-PAGE.

• Activity:

  Not Test

• Form:

  Liquid or Lyophilized powder

• Buffer:

  If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Function:

  In vitro phosphorylates phosphatidylglycerol but not diacylglycerol; the in vivo substrate is unknown.

• Molecular Weight:

  48 kDa

• References & Citations:

  "Expression, purification, crystallization and preliminary diffraction studies of the mammalian DAG kinase homologue yegS from Escherichia coli."Bakali H.M.A., Nordlund P., Hallberg B.M.Acta Crystallogr. F 62:295-297 (2006)

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

• Protein Length:

  Full Length