Recombinant Bovine Galectin-1 (LGALS1)

• Product Name Alternative:

  14KDA lectin Beta-galactoside-binding lectin L-14-I Galaptin Lactose-binding lectin 1 Lectin galactoside-binding soluble 1 S-Lac lectin 1

• Abbreviation:

  Recombinant Bovine LGALS1 protein

• Gene Name:

  LGALS1

• UniProt:

  P11116

• Expression Region:

  2-135aa

• Organism:

  Bos taurus (Bovine)

• Target Sequence:

  ACGLVASNLNLKPGECLRVRGEVAADAKSFLLNLGKDDNNLCLHFNPRFNAHGDVNTIVCNSKDAGAWGAEQRESAFPFQPGSVVEVCISFNQTDLTIKLPDGYEFKFPNRLNLEAINYLSAGGDFKIKCVAFE

• Tag:

  N-terminal 6xHis-SUMO-tagged

• Type:

  In Stock Protein

• Source:

  E.coli

• Field of Research:

  Cell Biology

• Relevance:

  Lectin that binds beta-galactoside and a wide array of complex carbohydrates (PubMed:1900835, PubMed:8108426, PubMed:7773775) . Plays a role in regulating apoptosis, cell proliferation and cell differentiation. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Strong inducer of T-cell apoptosis.

• Endotoxin:

  Not test

• Purity:

  Greater than 90% as determined by SDS-PAGE.

• Activity:

  Not Test

• Form:

  Liquid or Lyophilized powder

• Buffer:

  If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Function:

  Lectin that binds beta-galactoside and a wide array of complex carbohydrates

• Molecular Weight:

  30.6 kDa

• References & Citations:

  "Soluble bovine galactose-binding lectin. cDNA cloning reveals the complete amino acid sequence and an antigenic relationship with the major encephalitogenic domain of myelin basic protein."Abbott W.M., Mellor A., Edwards Y., Feizi T.Biochem. J. 259:283-290 (1989)

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

• Protein Length:

  Full Length of Mature Protein