Recombinant Human Granulocyte-macrophage colony-stimulating factor (CSF2) (Active)

• Product Name Alternative:

  Granulocyte-macrophage colony-stimulating factor; GM-CSF; Colony-stimulating factor (CSF) ; Molgramostin; Sargramostim

• Abbreviation:

  Recombinant Human CSF2 protein (Active)

• Gene Name:

  CSF2

• UniProt:

  P04141

• Expression Region:

  18-144aa

• Organism:

  Homo sapiens (Human)

• Target Sequence:

  APARSPSPSTQPWEHVNAIQEARRLLNLSRDTAAEMNETVEVISEMFDLQEPTCLQTRLELYKQGLRGSLTKLKGPLTMMASHYKQHCPPTPETSCATQIITFESFKENLKDFLLVIPFDCWEPVQE

• Tag:

  N-terminal 6xHis-tagged

• Type:

  Active Protein & In Stock Protein

• Source:

  Yeast

• Field of Research:

  Immunology

• Relevance:

  Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.

• Endotoxin:

  Less than 1.0 EU/μg as determined by LAL method.

• Purity:

  Greater than 95% as determined by SDS-PAGE.

• Activity:

  Yes

• Bioactivity:

  The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is 21.07-35.48 pg/mL.

• Form:

  Lyophilized powder

• Buffer:

  Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Function:

  Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.

• Molecular Weight:

  17.1 kDa

• References & Citations:

  GM-CSF Primes Proinflammatory Monocyte Responses in Ankylosing Spondylitis. Shi H., Chen L., Ridley A., Zaarour N., Brough I., Caucci C., Smith J.E., Bowness P. Front Immunol 11:1520-1520 (2020)

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

• Protein Length:

  Full Length of Mature Protein