Recombinant Dermatophagoides pteronyssinus Peptidase 1 (DERP1)

• Product Name Alternative:

  Allergen Der p I Major mite fecal allergen Der p 1 Allergen: Der p 1

• Abbreviation:

  Recombinant Dermatophagoides pteronyssinus DERP1 protein

• Gene Name:

  DERP1

• UniProt:

  P08176

• Expression Region:

  99-320aa

• Organism:

  Dermatophagoides pteronyssinus (European house dust mite)

• Target Sequence:

  TNACSINGNAPAEIDLRQMRTVTPIRMQGGCGSCWAFSGVAATESAYLAYRNQSLDLAEQELVDCASQHGCHGDTIPRGIEYIQHNGVVQESYYRYVAREQSCRRPNAQRFGISNYCQIYPPNVNKIREALAQTHSAIAVIIGIKDLDAFRHYDGRTIIQRDNGYQPNYHAVNIVGYSNAQGVDYWIVRNSWDTNWGDNGYGYFAANIDLMMIEEYPYVVIL

• Tag:

  N-terminal 6xHis-SUMO-tagged

• Type:

  In Stock Protein

• Source:

  E.coli

• Field of Research:

  Others

• Relevance:

  Thiol protease, with a preference for substrates with a large hydrophobic side chain in the P2 position, or with basic residues.

• Endotoxin:

  Not test

• Purity:

  Greater than 90% as determined by SDS-PAGE.

• Activity:

  Not Test

• Form:

  Liquid or Lyophilized powder

• Buffer:

  If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Function:

  Thiol protease, with a preference for substrates with a large hydrophobic side chain in the P2 position, or with basic residues.

• Molecular Weight:

  38.0 kDa

• References & Citations:

  "Cloning and expression of DNA coding for the major house dust mite allergen Der p 1 in Escherichia coli."Thomas W.R., Stewart G.A., Simpson R.J., Chua K.Y., Plozza T.M., Dilworth R.J., Nisbet A., Turner K.J.Int. Arch. Allergy Appl. Immunol. 85:127-129 (1988)

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

• Protein Length:

  Full Length of Mature Protein