Recombinant Human TATA box-binding protein-like protein 1 (TBPL1), partial

• Product Name Alternative:

  21KDA TBP-like protein; Second TBP of unique DNA protein ; STUDTATA box-binding protein-related factor 2 ; TBP-related factor 2TBP-like factorTBP-related protein

• Abbreviation:

  Recombinant Human TBPL1 protein, partial

• Gene Name:

  TBPL1

• UniProt:

  P62380

• Expression Region:

  1-185aa

• Organism:

  Homo sapiens (Human)

• Target Sequence:

  MDADSDVALDILITNVVCVFRTRCHLNLRKIALEGANVIYKRDVGKVLMKLRKPRITATIWSSGKIICTGATSEEEAKFGARRLARSLQKLGFQVIFTDFKVVNVLAVCNMPFEIRLPEFTKNNRPHASYEPELHPAVCYRIKSLRATLQIFSTGSITVTGPNVKAVATAVEQIYPFVFESRKEI

• Tag:

  N-terminal 6xHis-SUMO-tagged

• Type:

  Developed Protein

• Source:

  E.coli

• Field of Research:

  Epigenetics and Nuclear Signaling

• Relevance:

  Part of a specialized transcription syst that mediates the transcription of most ribosomal proteins through the 5'-TCT-3' motif which is a core promoter elent at these genes. Ses to also mediate the transcription of NF1. Does not bind the TATA box.

• Endotoxin:

  Not test

• Purity:

  Greater than 90% as determined by SDS-PAGE.

• Activity:

  Not Test

• Form:

  Liquid or Lyophilized powder

• Buffer:

  If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Function:

  Part of a specialized transcription system that mediates the transcription of most ribosomal proteins through the 5'-TCT-3' motif which is a core promoter element at these genes. Seems to also mediate the transcription of NF1. Does not bind the TATA box.

• Molecular Weight:

  36.8 kDa

• References & Citations:

  TRF2, but not TBP, mediates the transcription of ribosomal protein genes.Wang Y.L., Duttke S.H., Chen K., Johnston J., Kassavetis G.A., Zeitlinger J., Kadonaga J.T.Genes Dev. 28:1550-1555 (2014)

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

• Protein Length:

  Partial